The star of the beloved comedy came out on top Sunday.
The star of the since-ended comedy, Schitt's Creek, won the Globe for Best Actress in a Television Series, Musical or Comedy, during the bicoastal virtual awards show hosted by Tina Fey (from New York City's Rainbow Room) and Amy Poehler (from Beverly Hills' The Beverly Hilton ballroom), on Sunday.
O'Hara, who was never nominated at the Golden Globes before this year, took home the award for her thrilling performance as Moira Rose, a former soap star with a penchant for fabulous outfits.
The 66-year-old actress, who recently won an Emmy for the same performance in September, beat out fellow nominees Emily in Paris' Lily Collins, The Great's Elle Fanning, The Flight Attendant's Kaley Cuoco and Zoey's Extraordinary Playlist's Jane Levy.
During O'Hara's virtual acceptance speech, she and her husband, Bo Welch, took part in a comedy gag where he played off what was presumed to be "play-off" music as she was saying her thanks to the Schitt's Creek crew and intentionally made it difficult to hear her.
O'Hara congratulated her fellow nominees for being on the shortlist and expressed that she was "happily and seriously indebted" to Daniel and Eugene Levy, both Globe nominees.
"From Day 1, they treated me like something like this might happen," she said. "They created an inspiring, funny, beautiful family love story in which they let me wear a hundred wigs and speak like an alien. From the first read-through where no one had to fake a laugh to the final day on set where everybody, except me, couldn't hold back their tears. It's an experience I will forever hold dear to my heart. And I'm proud to be part of their family."
As O'Hara continued on with her speech, the "play-off" music began to play via her husband's phone and she pretended to rush through the rest of her kudos, breaking out into song to close it all out.
To stay up to date on breaking news, sign up for ET's daily newsletter.